Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX630657 - Provider product page
- Provider
- GeneTex
- Product name
- Glutamine synthetase antibody [GT7711]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract10 % SDS-PAGEGlutamine synthetase antibody [GT7711] (GTX630657) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis.A. 50 ?g mouse brain lysate/extract10 % SDS-PAGEGlutamine synthetase antibody [GT7711] (GTX630657) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis.A. 50 ?g rat brain lysate/extract10 % SDS-PAGEGlutamine synthetase antibody [GT7711] (GTX630657) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630657) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250.Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
