LF-MA0100

antibody from Invitrogen Antibodies

Targeting: GAPDHS GAPD2, GAPDH-2, GAPDS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LF-MA0100

Provider

Invitrogen Antibodies

Product name

Anti-GAPDH Monoclonal Antibody (4A1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

A suggested positive control for this product is HeLa cells treated with H2O2.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4A1

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Nuclear complex of glyceraldehyde-3-phosphate dehydrogenase and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxidant-induced cell death.

Hou X, Snarski P, Higashi Y, Yoshida T, Jurkevich A, Delafontaine P, Sukhanov S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jul;31(7):3179-3192

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1) and SH-SY5Y treated with Hydrogen Peroxide (1 mM Hydrogen Peroxide for 30 minutes). The blot was probed with Anti-GAPDH Mouse Monoclonal Antibody (Product # LF-MA0100, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 37 kDa band corresponding to GAPDH was observed upon treatment with Hydrogen Peroxide. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).