NBP1-47339

antibody from Novus Biologicals

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47339

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47339, RRID:AB_10010294

Product name

Mouse Monoclonal GAPDH Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Androgen receptor dampens tissue factor expression via nuclear factor-κB and early growth response protein 1.

Hoesel B, Mussbacher M, Dikorman B, Salzmann M, Assinger A, Hell L, Thaler J, Basílio J, Moser B, Resch U, Paar H, Mackman N, Schmid JA
Journal of thrombosis and haemostasis : JTH 2018 Apr;16(4):749-758

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Peroxisome proliferator-activated receptor-γ inhibits pancreatic cancer cell invasion and metastasis via regulating MMP-2 expression through PTEN.

Li Y, Zhang DW, Lin DQ, Cao LQ
Molecular medicine reports 2015 Oct;12(4):6255-60

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Nodal activates smad and extracellular signal-regulated kinases 1/2 pathways promoting renal cell carcinoma proliferation.

Zhang Z, Jiang T, Li Q, Wang J, Yang D, Li X, Wang Q, Song X
Molecular medicine reports 2015 Jul;12(1):587-94

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The Parkinson's-associated protein DJ-1 regulates the 20S proteasome.

Moscovitz O, Ben-Nissan G, Fainer I, Pollack D, Mizrachi L, Sharon M
Nature communications 2015 Apr 2;6:6609

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Dynamic regulation of the COP9 signalosome in response to DNA damage.

Füzesi-Levi MG, Ben-Nissan G, Bianchi E, Zhou H, Deery MJ, Lilley KS, Levin Y, Sharon M
Molecular and cellular biology 2014 Mar;34(6):1066-76

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.