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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [53]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
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- Product number
- NB300-328 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-328, RRID:AB_10000531
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Transcriptomic profiling reveals p53 as a key regulator of doxorubicin-induced cardiotoxicity.
The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer.
Mechanical Stabilization of the Glandular Acinus by Linker of Nucleoskeleton and Cytoskeleton Complex.
Oncostatin M exerts a protective effect against excessive scarring by counteracting the inductive effect of TGFβ1 on fibrosis markers.
The anti-inflammatory function of adenine occurs through AMPK activation and its downstream transcriptional regulation in THP-1 cells.
GAP31 from an ancient medicinal plant exhibits anti-viral activity through targeting to Epstein-Barr virus nuclear antigen 1.
Identification of microRNA-124 in regulation of Hepatocellular carcinoma through BIRC3 and the NF-κB pathway.
High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats.
Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer.
Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis.
FMRP recruitment of β-catenin to the translation pre-initiation complex represses translation.
Tubulin couples death receptor 5 to regulate apoptosis.
Zoledronate can induce colorectal cancer microenvironment expressing BTN3A1 to stimulate effector γδ T cells with antitumor activity.
MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer.
Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion.
Riluzole synergizes with paclitaxel to inhibit cell growth and induce apoptosis in triple-negative breast cancer.
Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes.
UV radiation promotes melanoma dissemination mediated by the sequential reaction axis of cathepsins-TGF-β1-FAP-α.
2-(Phenylsulfonyl)quinoline N-hydroxyacrylamides as potent anticancer agents inhibiting histone deacetylase.
Riluzole mediates anti-tumor properties in breast cancer cells independent of metabotropic glutamate receptor-1.
Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation.
Interleukin-17 potently increases non-small cell lung cancer growth.
Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance.
Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates.
Human mutations in methylenetetrahydrofolate dehydrogenase 1 impair nuclear de novo thymidylate biosynthesis.
Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.
Deubiquitinating enzymes regulate PARK2-mediated mitophagy.
Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer.
Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer.
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma.
Spreading of amyloid-β peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance.
Cortical synaptic NMDA receptor deficits in α7 nicotinic acetylcholine receptor gene deletion models: implications for neuropsychiatric diseases.
MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma.
A unifying mechanism for cancer cell death through ion channel activation by HAMLET.
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
Difference in protein expression profile and chemotherapy drugs response of different progression stages of LNCaP sublines and other human prostate cancer cells.
Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by Gefitinib and Cetuximab triggered antibody-dependent cellular cytotoxicity.
Shear stress modulates VCAM-1 expression in response to TNF-α and dietary lipids via interferon regulatory factor-1 in cultured endothelium.
Modulation of airway epithelial cell functions by Pidotimod: NF-kB cytoplasmatic expression and its nuclear translocation are associated with an increased TLR-2 expression.
Spinal serum-inducible and glucocorticoid-inducible kinase 1 mediates neuropathic pain via kalirin and downstream PSD-95-dependent NR2B phosphorylation in rats.
Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell.
Celecoxib induces proliferation and Amphiregulin production in colon subepithelial myofibroblasts, activating erk1-2 signaling in synergy with EGFR.
Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin β1.
Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers.
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Shear stress modulates RAGE-mediated inflammation in a model of diabetes-induced metabolic stress.
Regulation of neuroendocrine differentiation by AKT/hnRNPK/AR/β-catenin signaling in prostate cancer cells.
Upregulation of cellular Bcl-2 by the KSHV encoded RTA promotes virion production.
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.
Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells.
McSweeney KM, Bozza WP, Alterovitz WL, Zhang B
Cell death discovery 2019;5:102
Cell death discovery 2019;5:102
The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer.
Melissaridou S, Wiechec E, Magan M, Jain MV, Chung MK, Farnebo L, Roberg K
Cancer cell international 2019;19:16
Cancer cell international 2019;19:16
Mechanical Stabilization of the Glandular Acinus by Linker of Nucleoskeleton and Cytoskeleton Complex.
Zhang Q, Narayanan V, Mui KL, O'Bryan CS, Anderson RH, Kc B, Cabe JI, Denis KB, Antoku S, Roux KJ, Dickinson RB, Angelini TE, Gundersen GG, Conway DE, Lele TP
Current biology : CB 2019 Sep 9;29(17):2826-2839.e4
Current biology : CB 2019 Sep 9;29(17):2826-2839.e4
Oncostatin M exerts a protective effect against excessive scarring by counteracting the inductive effect of TGFβ1 on fibrosis markers.
Huguier V, Giot JP, Simonneau M, Levillain P, Charreau S, Garcia M, Jégou JF, Bodet C, Morel F, Lecron JC, Favot L
Scientific reports 2019 Feb 14;9(1):2113
Scientific reports 2019 Feb 14;9(1):2113
The anti-inflammatory function of adenine occurs through AMPK activation and its downstream transcriptional regulation in THP-1 cells.
Wu TT, Chen CC, Lin JT, Young GH, Wang HC, Chen HM
Bioscience, biotechnology, and biochemistry 2019 Aug 8;:1-10
Bioscience, biotechnology, and biochemistry 2019 Aug 8;:1-10
GAP31 from an ancient medicinal plant exhibits anti-viral activity through targeting to Epstein-Barr virus nuclear antigen 1.
Shen CL, Huang WH, Hsu HJ, Yang JH, Peng CW
Antiviral research 2019 Apr;164:123-130
Antiviral research 2019 Apr;164:123-130
Identification of microRNA-124 in regulation of Hepatocellular carcinoma through BIRC3 and the NF-κB pathway.
Cao J, Qiu J, Wang X, Lu Z, Wang D, Feng H, Li X, Liu Q, Pan H, Han X, Wei J, Liu S, Wang L
Journal of Cancer 2018;9(17):3006-3015
Journal of Cancer 2018;9(17):3006-3015
High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats.
Carrasco-Ruiz MLÁ, Hernández-Aragón LG, Chávez-Ríos JR, Rodríguez-Antolín J, Pacheco P, Martínez-Gómez M, Cuevas-Romero E, Castelán F
International neurourology journal 2018 Sep;22(3):161-168
International neurourology journal 2018 Sep;22(3):161-168
Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer.
Sexton RE, Hachem AH, Assi AA, Bukhsh MA, Gorski DH, Speyer CL
Scientific reports 2018 Oct 30;8(1):16008
Scientific reports 2018 Oct 30;8(1):16008
Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis.
Bozza WP, Zhang Y, Zhang B
Oncotarget 2018 May 1;9(33):23264-23273
Oncotarget 2018 May 1;9(33):23264-23273
FMRP recruitment of β-catenin to the translation pre-initiation complex represses translation.
Ehyai S, Miyake T, Williams D, Vinayak J, Bayfield MA, McDermott JC
EMBO reports 2018 Dec;19(12)
EMBO reports 2018 Dec;19(12)
Tubulin couples death receptor 5 to regulate apoptosis.
Twomey JD, Zhao L, Luo S, Xu Q, Zhang B
Oncotarget 2018 Dec 4;9(95):36804-36815
Oncotarget 2018 Dec 4;9(95):36804-36815
Zoledronate can induce colorectal cancer microenvironment expressing BTN3A1 to stimulate effector γδ T cells with antitumor activity.
Zocchi MR, Costa D, Venè R, Tosetti F, Ferrari N, Minghelli S, Benelli R, Scabini S, Romairone E, Catellani S, Profumo A, Poggi A
Oncoimmunology 2017;6(3):e1278099
Oncoimmunology 2017;6(3):e1278099
MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer.
Cao J, Yan XR, Liu T, Han XB, Yu JJ, Liu SH, Wang LB
Oncology letters 2017 Sep;14(3):3795-3802
Oncology letters 2017 Sep;14(3):3795-3802
Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion.
Bulanova DR, Akimov YA, Rokka A, Laajala TD, Aittokallio T, Kouvonen P, Pellinen T, Kuznetsov SG
Cell adhesion & migration 2017 Sep 3;11(5-6):434-446
Cell adhesion & migration 2017 Sep 3;11(5-6):434-446
Riluzole synergizes with paclitaxel to inhibit cell growth and induce apoptosis in triple-negative breast cancer.
Speyer CL, Bukhsh MA, Jafry WS, Sexton RE, Bandyopadhyay S, Gorski DH
Breast cancer research and treatment 2017 Nov;166(2):407-419
Breast cancer research and treatment 2017 Nov;166(2):407-419
Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes.
Zhao L, Zhang B
Scientific reports 2017 Mar 16;7:44735
Scientific reports 2017 Mar 16;7:44735
UV radiation promotes melanoma dissemination mediated by the sequential reaction axis of cathepsins-TGF-β1-FAP-α.
Wäster P, Orfanidis K, Eriksson I, Rosdahl I, Seifert O, Öllinger K
British journal of cancer 2017 Aug 8;117(4):535-544
British journal of cancer 2017 Aug 8;117(4):535-544
2-(Phenylsulfonyl)quinoline N-hydroxyacrylamides as potent anticancer agents inhibiting histone deacetylase.
Lee HY, Chang CY, Su CJ, Huang HL, Mehndiratta S, Chao YH, Hsu CM, Kumar S, Sung TY, Huang YZ, Li YH, Yang CR, Liou JP
European journal of medicinal chemistry 2016 Oct 21;122:92-101
European journal of medicinal chemistry 2016 Oct 21;122:92-101
Riluzole mediates anti-tumor properties in breast cancer cells independent of metabotropic glutamate receptor-1.
Speyer CL, Nassar MA, Hachem AH, Bukhsh MA, Jafry WS, Khansa RM, Gorski DH
Breast cancer research and treatment 2016 Jun;157(2):217-228
Breast cancer research and treatment 2016 Jun;157(2):217-228
Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation.
Bergström P, Agholme L, Nazir FH, Satir TM, Toombs J, Wellington H, Strandberg J, Bontell TO, Kvartsberg H, Holmström M, Boreström C, Simonsson S, Kunath T, Lindahl A, Blennow K, Hanse E, Portelius E, Wray S, Zetterberg H
Scientific reports 2016 Jul 7;6:29200
Scientific reports 2016 Jul 7;6:29200
Interleukin-17 potently increases non-small cell lung cancer growth.
Wei L, Wang H, Yang F, Ding Q, Zhao J
Molecular medicine reports 2016 Feb;13(2):1673-80
Molecular medicine reports 2016 Feb;13(2):1673-80
Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance.
Carta S, Penco F, Lavieri R, Martini A, Dinarello CA, Gattorno M, Rubartelli A
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):2835-40
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):2835-40
Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates.
Cuellar TL, Barnes D, Nelson C, Tanguay J, Yu SF, Wen X, Scales SJ, Gesch J, Davis D, van Brabant Smith A, Leake D, Vandlen R, Siebel CW
Nucleic acids research 2015 Jan;43(2):1189-203
Nucleic acids research 2015 Jan;43(2):1189-203
Human mutations in methylenetetrahydrofolate dehydrogenase 1 impair nuclear de novo thymidylate biosynthesis.
Field MS, Kamynina E, Watkins D, Rosenblatt DS, Stover PJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 13;112(2):400-5
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 13;112(2):400-5
Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.
Lin YS, Yang CC, Hsu CC, Hsu JT, Wu SC, Lin CJ, Cheng WT
Transgenic research 2015 Feb;24(1):61-71
Transgenic research 2015 Feb;24(1):61-71
Deubiquitinating enzymes regulate PARK2-mediated mitophagy.
Wang Y, Serricchio M, Jauregui M, Shanbhag R, Stoltz T, Di Paolo CT, Kim PK, McQuibban GA
Autophagy 2015 Apr 3;11(4):595-606
Autophagy 2015 Apr 3;11(4):595-606
Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer.
Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH
PloS one 2014;9(3):e88830
PloS one 2014;9(3):e88830
Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer.
Banda M, Speyer CL, Semma SN, Osuala KO, Kounalakis N, Torres Torres KE, Barnard NJ, Kim HJ, Sloane BF, Miller FR, Goydos JS, Gorski DH
PloS one 2014;9(1):e81126
PloS one 2014;9(1):e81126
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
Venè R, Cardinali B, Arena G, Ferrari N, Benelli R, Minghelli S, Poggi A, Noonan DM, Albini A, Tosetti F
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma.
Salcher S, Hagenbuchner J, Geiger K, Seiter MA, Rainer J, Kofler R, Hermann M, Kiechl-Kohlendorfer U, Ausserlechner MJ, Obexer P
Molecular cancer 2014 Sep 28;13:224
Molecular cancer 2014 Sep 28;13:224
Spreading of amyloid-β peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance.
Domert J, Rao SB, Agholme L, Brorsson AC, Marcusson J, Hallbeck M, Nath S
Neurobiology of disease 2014 May;65:82-92
Neurobiology of disease 2014 May;65:82-92
Cortical synaptic NMDA receptor deficits in α7 nicotinic acetylcholine receptor gene deletion models: implications for neuropsychiatric diseases.
Lin H, Hsu FC, Baumann BH, Coulter DA, Lynch DR
Neurobiology of disease 2014 Mar;63:129-40
Neurobiology of disease 2014 Mar;63:129-40
MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma.
Poenitzsch Strong AM, Setaluri V, Spiegelman VS
Archives of biochemistry and biophysics 2014 Dec 1;563:118-24
Archives of biochemistry and biophysics 2014 Dec 1;563:118-24
A unifying mechanism for cancer cell death through ion channel activation by HAMLET.
Storm P, Klausen TK, Trulsson M, Ho C S J, Dosnon M, Westergren T, Chao Y, Rydström A, Yang H, Pedersen SF, Svanborg C
PloS one 2013;8(3):e58578
PloS one 2013;8(3):e58578
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
Borghgraef P, Menuet C, Theunis C, Louis JV, Devijver H, Maurin H, Smet-Nocca C, Lippens G, Hilaire G, Gijsen H, Moechars D, Van Leuven F
PloS one 2013;8(12):e84442
PloS one 2013;8(12):e84442
Difference in protein expression profile and chemotherapy drugs response of different progression stages of LNCaP sublines and other human prostate cancer cells.
Lin HP, Lin CY, Hsiao PH, Wang HD, Jiang SS, Hsu JM, Jim WT, Chen M, Kung HJ, Chuu CP
PloS one 2013;8(12):e82625
PloS one 2013;8(12):e82625
Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
Wang YI, Bettaieb A, Sun C, DeVerse JS, Radecke CE, Mathew S, Edwards CM, Haj FG, Passerini AG, Simon SI
PloS one 2013;8(10):e78322
PloS one 2013;8(10):e78322
Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by Gefitinib and Cetuximab triggered antibody-dependent cellular cytotoxicity.
Amaro A, Mirisola V, Angelini G, Musso A, Tosetti F, Esposito AI, Perri P, Lanza F, Nasciuti F, Mosci C, Puzone R, Salvi S, Truini M, Poggi A, Pfeffer U
European journal of cancer (Oxford, England : 1990) 2013 Oct;49(15):3353-65
European journal of cancer (Oxford, England : 1990) 2013 Oct;49(15):3353-65
Shear stress modulates VCAM-1 expression in response to TNF-α and dietary lipids via interferon regulatory factor-1 in cultured endothelium.
DeVerse JS, Sandhu AS, Mendoza N, Edwards CM, Sun C, Simon SI, Passerini AG
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1149-57
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1149-57
Modulation of airway epithelial cell functions by Pidotimod: NF-kB cytoplasmatic expression and its nuclear translocation are associated with an increased TLR-2 expression.
Carta S, Silvestri M, Rossi GA
Italian journal of pediatrics 2013 May 10;39:29
Italian journal of pediatrics 2013 May 10;39:29
Spinal serum-inducible and glucocorticoid-inducible kinase 1 mediates neuropathic pain via kalirin and downstream PSD-95-dependent NR2B phosphorylation in rats.
Peng HY, Chen GD, Lai CY, Hsieh MC, Lin TB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5227-40
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5227-40
Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell.
Benelli R, Stigliani S, Minghelli S, Carlone S, Ferrari N
The Prostate 2013 Jun;73(9):941-51
The Prostate 2013 Jun;73(9):941-51
Celecoxib induces proliferation and Amphiregulin production in colon subepithelial myofibroblasts, activating erk1-2 signaling in synergy with EGFR.
Benelli R, Venè R, Minghelli S, Carlone S, Gatteschi B, Ferrari N
Cancer letters 2013 Jan 1;328(1):73-82
Cancer letters 2013 Jan 1;328(1):73-82
Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin β1.
Shin M, Mizokami A, Kim J, Ofude M, Konaka H, Kadono Y, Kitagawa Y, Miwa S, Kumaki M, Keller ET, Namiki M
The Prostate 2013 Aug;73(11):1159-70
The Prostate 2013 Aug;73(11):1159-70
Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers.
Loke WM, Lam KM, Chong WL, Chew SE, Quek AM, Lim ECh, Seet RC
Free radical research 2012 Oct;46(10):1230-7
Free radical research 2012 Oct;46(10):1230-7
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
Innate immunity 2012 Jun;18(3):531-40
Innate immunity 2012 Jun;18(3):531-40
Shear stress modulates RAGE-mediated inflammation in a model of diabetes-induced metabolic stress.
DeVerse JS, Bailey KA, Jackson KN, Passerini AG
American journal of physiology. Heart and circulatory physiology 2012 Jun 15;302(12):H2498-508
American journal of physiology. Heart and circulatory physiology 2012 Jun 15;302(12):H2498-508
Regulation of neuroendocrine differentiation by AKT/hnRNPK/AR/β-catenin signaling in prostate cancer cells.
Ciarlo M, Benelli R, Barbieri O, Minghelli S, Barboro P, Balbi C, Ferrari N
International journal of cancer 2012 Aug 1;131(3):582-90
International journal of cancer 2012 Aug 1;131(3):582-90
Upregulation of cellular Bcl-2 by the KSHV encoded RTA promotes virion production.
Gao J, Cai Q, Lu J, Jha HC, Robertson ES
PloS one 2011;6(8):e23892
PloS one 2011;6(8):e23892
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Field MS, Anderson DD, Stover PJ
Frontiers in genetics 2011;2:36
Frontiers in genetics 2011;2:36
Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.
Johnen S, Kazanskaya O, Armogan N, Stickelmann C, Stöcker M, Walter P, Thumann G
Investigative ophthalmology & visual science 2011 Jul 23;52(8):5400-7
Investigative ophthalmology & visual science 2011 Jul 23;52(8):5400-7
Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells.
Cheng G, Shao Z, Chaudhari B, Agrawal DK
American journal of physiology. Lung cellular and molecular physiology 2007 Nov;293(5):L1339-47
American journal of physiology. Lung cellular and molecular physiology 2007 Nov;293(5):L1339-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (2D4A7) [NB300-328] - HeLa cell lysate using GAPDH antibody (NB300-328) at 1:1000. Theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody (2D4A7) [NB300-328] - Image shows a specific band for GAPDH in 0.05 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (2D4A7) [NB300-328] - Analysis using anti-GAPDH monoclonal antibody, against cell lysate (2: HeLa, 3: K562, 4: Jurkat, 5: NIH/3T3, 6: HepG-2). Note: band observed higher than predicted molecular weight of 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (2D4A7) [NB300-328] - Detection of GAPDH in mouse liver. Note: band observed higher than predicted molecular weight of 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (2D4A7) [NB300-328] - Lysates of HeLa human cervical epithelial carcinoma cell line loaded at the indicated concentrations. PVDF membrane was probed with 1:20000 dilution of mouse anti-GAPDH monoclonal antibody (NB300-328) followed by 1:2000 dilution of donkey anti-mouse IgG-HRP secondary antibody (HAF018).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (2D4A7) [NB300-328] - Analysis using the HRP conjugate of NB300-328. Detection of Lysosome-associated membrane protein 2 (LAMP-2) upregulation with intracellular amyloid-B accumulations quantified against GAPDH. Image from verfied customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) [NB300-328] - Confocal immunofluorescence analysis of HeLa cells using GAPDH antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) [NB300-328] - Confocal analysis of HeLa cells using GAPDH antibody (NB300-328, 1:50). An Alexa Fluor 488-conjugated Goat anti mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) [NB300-328] - Detection of GAPDH (Green) in HeLa cells using NB300-328 at a 1:200 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) [NB300-328] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328, Novus Biologicals), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339, Novus Biologicals). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (2D4A7) [NB300-328] - Analysis using paraffin-embedded human lung cancer tissue with GAPDH Antibody at a dilution of 1:50. Detection was completed using VC001 (DAB) and counterstained by hematoxylin. Labeling is predominantly in epithelial cells.
