NB300-323

antibody from Novus Biologicals

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB300-323

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-323, RRID:AB_10003259

Product name

Rabbit Polyclonal GAPDH Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Xenopus, Yeast

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody [NB300-323] - Detection of Human and Mouse GAPDH, theoretical molecular weight of 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Another Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/mL. Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody [NB300-323] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657]. Note: band observed higher than predicted molecular weight of 36 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody [NB300-323] - Western blot analysis of GAPDH (NB300-323) using NIH3T3 whole cell lysate [NB800-PC8]. Theoretical molecular weight: 36 kDa

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: GAPDH Antibody [NB300-323] - Simple Western lane view shows a specific band for GAPDH in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.