AF248

antibody from Novus Biologicals

Targeting: BDNF

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF248

Provider

Novus Biologicals

Product name

Chicken Polyclonal BDNF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human BDNF in direct ELISAs.

Reactivity

Human

Host

Chicken/Avian

Isotype

IgY

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Acid fibroblast growth factor and peripheral nerve grafts regulate Th2 cytokine expression, macrophage activation, polyamine synthesis, and neurotrophin expression in transected rat spinal cords.

Kuo HS, Tsai MJ, Huang MC, Chiu CW, Tsai CY, Lee MJ, Huang WC, Lin YL, Kuo WC, Cheng H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Mar 16;31(11):4137-47

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Brain-derived neurotrophic factor mediates non-cell-autonomous regulation of sensory neuron position and identity.

Wright MA, Ribera AB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Oct 27;30(43):14513-21

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PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex.

Ohtsuka M, Fukumitsu H, Furukawa S
Biochemical and biophysical research communications 2008 May 16;369(4):1144-9

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Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.

Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
The European journal of neuroscience 2008 Apr;27(8):2089-102

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BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.

Hu B, Nikolakopoulou AM, Cohen-Cory S
Development (Cambridge, England) 2005 Oct;132(19):4285-98

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) at 15 µg/mL overnight at 4 °C. Tissue was stained using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.