H00000627-D01P

antibody from Abnova Corporation

Targeting: BDNF

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000627-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000627-D01P, RRID:AB_1571768

Product name

BDNF purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human BDNF protein.

Antigen sequence

MFHQVRRVMTILFLTMVISYFGCMKAAPMKEANIR
GQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADT
FEHVIEELLDEDQKVRPNEENNKDADLYTSRVMLS
SQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDP
ARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVL
EKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKR
HWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSC
VCTLTIKRGR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BDNF expression in transfected 293T cell line (H00000627-T02) by BDNF MaxPab polyclonal antibody.Lane 1: BDNF transfected lysate(28.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between BDNF and NTF4. HeLa cells were stained with anti-BDNF rabbit purified polyclonal 1:1200 and anti-NTF4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)