Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOCS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, mouse brain, HOK, DOK.
- Concentration
- 1 mg/mL
Submitted references MicroRNA‑19b inhibitors can attenuate the STAT3 signaling pathway in NPC C666‑1 cells.
Immune promotive effect of bioactive peptides may be mediated by regulating the expression of SOCS1/miR-155.
Bian LH, Duan JL, Zhou C, Shen GW, Wang XY, Yang Y, Zhang XL, Xiao SJ
Molecular medicine reports 2020 Jul;22(1):51-56
Molecular medicine reports 2020 Jul;22(1):51-56
Immune promotive effect of bioactive peptides may be mediated by regulating the expression of SOCS1/miR-155.
Chen C, Su X, Hu Z
Experimental and therapeutic medicine 2019 Sep;18(3):1850-1862
Experimental and therapeutic medicine 2019 Sep;18(3):1850-1862
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOCS1 Polyclonal Antibody (Product # PA5-27239). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 12% SDS PAGE. SOCS1 Polyclonal Antibody (Product # PA5-27239) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOCS1 Polyclonal Antibody detects SOCS1 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract.12 % SDS-PAGE. SOCS1 Polyclonal Antibody (Product # PA5-27239) dilution: 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SOCS1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SOCS1 Polyclonal Antibody (Product # PA5-27239) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SOCS1 (Product # PA5-27239) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOCS1 Polyclonal Antibody detects SOCS1 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SOCS1 Polyclonal Antibody (Product # PA5-27239) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
