MA1-41219

antibody from Invitrogen Antibodies

Targeting: IKBKG FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9

Antibody data

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Validation data

Product number: MA1-41219 - Provider product page
Provider: Invitrogen Antibodies
Product name: IKK gamma Monoclonal Antibody (46B844)
Antibody type: Monoclonal
Antigen: Other
Description: Suggested positive control: Daudi or Jurkat.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 46B844
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

IKBKG protein structure - MA1-41219 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using a IKK gamma monoclonal antibody (Product # MA1-41219). Lysate from human Jurkat cells probed with a IKK gamma monoclonal antibody (Product # MA1-41219) at 2 µg/mL. Goat anti-mouse Ig HRP secondary.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of IKK gamma in lysate from human Jurkat cells. Samples were incubated in IKK gamma monoclonal antibody (Product # MA1-41219) using a dilution of 2 µg/mL followed by a goat anti-mouse IgG1 HRP secondary antibody. PicoTect ECL substrate solution was used for this test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of IKKG was achieved by transfecting HEK-293 cells with IKKG specific siRNA (Silencer® select Product # s16186). Western blot analysis (Fig. a) was performed using whole cell extracts from the IKKG knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with IKKG Monoclonal Antibody (Product # MA1-41219, 2 µg/mL) and chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to IKKG. Few uncharacterized bands (*) were observed between 30-40 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-IKKG Polyclonal Antibody(Product # MA1-41219) a 48 kDa band corresponding to IKKG was observed across the cell lines tested. Additional un-characterized bands (*) were observed (a band around 36 kDa could be the probable isoform of IKKG). Whole cell extracts of (30 µg lysate) of U-87MG (Lane 1), HEK-293 (Lane 2), MCF-7 (Lane 3), Daudi (Lane 4), HeLa (Lane 5), PC-3 (Lane 6) and Jurkat (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).