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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB7616 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha 2b/CD41 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin alpha 2b/CD41 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 5, alpha 8, alpha V, beta 3, beta 5, beta 6, recombinant mouse Integrin beta 2b or beta 3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 745201
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Integrin alpha 2b/CD41 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin alpha 2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin alpha 2b/CD41 (Clone P2) and either (A) Mouse IgG1 Isotype Control (Catalog # MAB002) or (B) Mouse Anti-Human Integrin alpha 2b/CD41 Mono-clonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.
