A15454

antibody from Invitrogen Antibodies

Targeting: CD33 FLJ00391, p67, SIGLEC-3, SIGLEC3

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A15454

Provider

Invitrogen Antibodies

Product name

CD33 Monoclonal Antibody (WM53), PE-Cyanine5.5

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

WM53

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Imidazo[1,2-b]pyrazole-7-Carboxamide Derivative Induces Differentiation-Coupled Apoptosis of Immature Myeloid Cells Such as Acute Myeloid Leukemia and Myeloid-Derived Suppressor Cells.

Kotogány E, Balog JÁ, Nagy LI, Alföldi R, Bertagnolo V, Brugnoli F, Demjén A, Kovács AK, Batár P, Mezei G, Szabó R, Kanizsai I, Varga C, Puskás LG, Szebeni GJ
International journal of molecular sciences 2020 Jul 20;21(14)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD33 in human peripheral blood granulocytes. Cells were stained using CD33 Monoclonal Antibody (WM53), PE-Cyanine5.5 (Product # A15454).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 The human 'AML1' patient-derived bone marrow aspirate cells responded to DU325 with differentiation-coupled apoptosis. The loss of the percentage of CD33 positive and an increase of CD11b positive cells were assayed by flow cytometry ( A ) and ( B ) after 48 incubation with 200 nM DU325. The median fluorescent intensity (MFI) proportional with CD33 expression on the cells also decreased by 200 nM DU325 ( C ) and ( D ). The resazurin viability assay determined IC 50 value of DU325 on the primary human patient-derived 'AML1' cells after 72 h treatment of 80.6 nM. Concentrations of DU325 were as follows, 3 muM, 1muM, 333 nM, 111 nM, 37 nM, and 12 nM ( E ). The combined Annexin V and propidium iodide (AnnV/PI) flow cytometry staining showed an increase of both AnnV+/PI- early and AnnV+/PI+ late apoptotic cells after treatment with 200 nM DU325 for 48 h ( F ). Data are shown as arithmetic mean values +- standard deviation. Statistical significance was set to *** p < 0.001.