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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- UM500043 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500043, RRID:AB_2629057
- Product name
- L1CAM mouse monoclonal antibody, clone UMAB47
- Antibody type
- Monoclonal
- Description
- L1CAM mouse monoclonal antibody, clone UMAB47
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- L1CAM
- Isotype
- IgG
- Antibody clone number
- UMAB47
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from HeLa. Diluation: 1:500
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluoresce image of primary rat neurons labeled with anti-L1CAM mouse monoclonal antibody (UM500043, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-L1CAM mouse monoclonal antibody. (UM500043; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-L1CAM mouse monoclonal antibody. (UM500043; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pituitary using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows only a subset of cells with positive cytoplasmic and membraneous stain.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovcarian carcinoma using anti-L1CAM clone UMAB47 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500043); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas using anti-L1CAM clone UMAB47 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500043); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen glandular cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining seen in staining in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human brain using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining seen in staining in granular layer and the molecular layer.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-L1CAM mouse monoclonal antibody (UM500043). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
