GTX84235

antibody from GeneTex

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84235

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84235, RRID:AB_10729436

Product name

L1CAM antibody [2A5]

Antibody type

Monoclonal

Reactivity

Human, Simian

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-L1CAM antibody(GTX84235),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells transfected with either RC211601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-L1CAM antibody(GTX84235), and then analyzed by flow cytometry.