- UM500023 - OriGene CDH2 antibody

Submitted references Macrophages significantly enhance wound healing in a vascularized skin model.

Kreimendahl F, Marquardt Y, Apel C, Bartneck M, Zwadlo-Klarwasser G, Hepp J, Jockenhoevel S, Baron JM
Journal of biomedical materials research. Part A 2019 Jun;107(6):1340-1350

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Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western Blot analysis of extracts (35ug) from 11 different cell lines by using anti-CDH2 monoclonal antibody (Clone UMAB23)
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western blot of mouse tissue lysates (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot? Ultra (1:1000).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunofluorescent staining of HeLa cells using anti-CDH2 mouse monoclonal antibody (UM500023, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of 3 human carcinoma of kidney using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human carcinoma of the kidney all show strong membraneous and cytoplasmic staining in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human pancreas using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows gladular cells of the pancreas membraneous and cytoplasmic staining cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human lymph node using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows cells of the lymph node are negative. Positive staining seen in the endothelial cells, mostly membraneous with weak cytoplasmic .
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human carcinoma of the lung using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows the tumor cells with membraneous and cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human endometrium using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the endometrial cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human ovarian carcinoma using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human kidney using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining in the kidney tubules
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded of human thyroid carcinoma using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells
Validation comment: IHC

UM500023

Antibody data