TA500498

antibody from OriGene

Targeting: CTAG1B CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, NY-ESO-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

TA500498

Provider

OriGene

Proper citation

OriGene Cat#TA500498, RRID:AB_2292371

Product name

Purified CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI3F10 (formerly 3F10)

Antibody type

Monoclonal

Description

Purified CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI3F10 (formerly 3F10)

Host

Mouse

Conjugate

Unconjugated

Epitope

CTAG1B

Isotype

IgG

Antibody clone number

OTI3F10

Vial size

100 µl

Concentration

0.95 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CTAG1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using anti-CTAG1B mouse monoclonal antibody (TA500498).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CTAG1B mouse monoclonal antibody (TA500498) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTAG1B(RC213318).

Validation comment

IF