NBP1-47256

antibody from Novus Biologicals

Targeting: POLA1 NSX, p180, POLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-47256

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47256, RRID:AB_10010210

Product name

Rabbit Polyclonal DNA polymerase alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope