Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN954147 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Pinin, Desmosome Associated Protein (PNN) (AA 216-248), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 216-248 amino acids from the Central region of human PNN.
- Description
- Affinitty Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 216-248,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Pinin / PNN Antibody Cat.-No AP53368PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PNN antibody detected the PNN protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Pinin / PNN Antibody Cat.-No AP53368PU-N with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human liver tissue reacted with Pinin / PNN Antibody Cat.-No AP53368PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
