MA1-83256

antibody from Invitrogen Antibodies

Targeting: INS IDDM1, IDDM2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-83256

Provider

Invitrogen Antibodies

Product name

Insulin/Proinsulin Monoclonal Antibody (D3E7 (5B6/6))

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product has an Affinity of 6.3x10^8 M^-1.

Antibody clone number

D3E7 (5B6/6)

Concentration

1 mg/mL

References

Submitted references

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Merriman C, Fu D
The Journal of biological chemistry 2019 Nov 8;294(45):16992-17006

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Insulin/Proinsulin Monoclonal Antibody (Product # MA1-83256 ) and bands around 7, 10 kDa corresponding to Insulin/Proinsulin were observed. 1 µg of purified recombinant Human Insulin was electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human pancreas using a Insulin+Proinsulin monoclonal antibody (Product # MA1-83256).