IC1417A

antibody from Novus Biologicals

Targeting: INS IDDM1, IDDM2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1417A

Provider

Novus Biologicals

Product name

Rat Monoclonal Insulin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects bovine, human, and mouse insulin.

Reactivity

Human, Mouse, Bovine

Host

Rat

Conjugate

Red dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Merriman C, Fu D
The Journal of biological chemistry 2019 Nov 8;294(45):16992-17006

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microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9.

Xu Y, Huang Y, Guo Y, Xiong Y, Zhu S, Xu L, Lu J, Li X, Wan J, Lu Y, Wang Z
Stem cell research & therapy 2019 Feb 15;10(1):59

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Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes.

Dooley J, Tian L, Schonefeldt S, Delghingaro-Augusto V, Garcia-Perez JE, Pasciuto E, Di Marino D, Carr EJ, Oskolkov N, Lyssenko V, Franckaert D, Lagou V, Overbergh L, Vandenbussche J, Allemeersch J, Chabot-Roy G, Dahlstrom JE, Laybutt DR, Petrovsky N, Socha L, Gevaert K, Jetten AM, Lambrechts D, Linterman MA, Goodnow CC, Nolan CJ, Lesage S, Schlenner SM, Liston A
Nature genetics 2016 May;48(5):519-27

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Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.

Kornete M, Beauchemin H, Polychronakos C, Piccirillo CA
Autoimmunity 2013 Jun;46(4):259-68

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Insulin in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC-conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.