Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB02312 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- HIST1H2AL antibody - C-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HIST1H2AL Antibody (C-term) western blot analysis in HL-60,HepG2,K562,CEM,MCF-7 cell lysates (35ug/lane).This demonstrates the HIST1H2AL antibody detected the HIST1H2AL protein (arrow).
- Sample type
- HL-60,HepG2,K562,CEM,MCF-7 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HIST1H2AL Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2AL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human lung carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HIST1H2AL Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- HepG2 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol