ALX-805-039-C100

antibody from Enzo Life Sciences

Targeting: CD36 FAT, GP3B, GP4, GPIV, SCARB3

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Data presented on provider website)

Functional assay (Recommended by provider)

Antibody Data

Product number

ALX-805-039-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-805-039-C100, RRID:AB_2051040

Product name

CD36 monoclonal antibody (63)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Reactivity

Mouse, Rat

Host

Mouse

Isotype

IgA

Antibody clone number

63

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Murine insulin treated or untreated adipocytes fixed with acetone were incubated with murine anti-mouse CD36 antibody (1:100 dilution of supernatant), followed by FITC-conjugated IgA-specific secondary antibody. Confocal microscopy revealed prominent membrane staining (left panels). Phase contrast images are shown in the right panels.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Elicited mouse peritoneal macrophages were incubated with murine anti-mouse CD36 antibody (1:100 dilution of supernatant) or an isotype control (IgA), followed by FITC-conjugated IgA specific secondary antibody, and then analyzed by flow cytometry. The shaded peak shows high level expression of CD36.