AF1955

antibody from R&D Systems

Targeting: CD36 FAT, GP3B, GP4, GPIV, SCARB3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1955

Provider

R&D Systems

Product name

Human CD36/SR-B3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human CD36 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CD36 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P16671


Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis.

Ladanyi A, Mukherjee A, Kenny HA, Johnson A, Mitra AK, Sundaresan S, Nieman KM, Pascual G, Benitah SA, Montag A, Yamada SD, Abumrad NA, Lengyel E
Oncogene 2018 Apr;37(17):2285-2301

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Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation.

Samovski D, Sun J, Pietka T, Gross RW, Eckel RH, Su X, Stahl PD, Abumrad NA
Diabetes 2015 Feb;64(2):353-9

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A novel ELISA for measuring CD36 protein in human adipose tissue.

Allred CC, Krennmayr T, Koutsari C, Zhou L, Ali AH, Jensen MD
Journal of lipid research 2011 Feb;52(2):408-15

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Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.

Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circulation research 2009 Aug 28;105(5):500-10

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Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity.

Mahadevan VS, Campbell M, McKeown PP, Bayraktutan U
Cardiovascular research 2006 Oct 1;72(1):60-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CD36/SR-B3 by Western Blot. Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CD36/SR-B3 by Simple Western<SUP abp="261">TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CD36/SR-B3 by Simple Western^TM. Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.