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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0616 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0616, RRID:AB_982945
- Product name
- STAT3 (phospho Y705) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TEL/ETV6 is a signal transducer and activator of transcription 3 (Stat3)-induced repressor of Stat3 activity.
Proteolytic conversion of STAT3alpha to STAT3gamma in human neutrophils: role of granule-derived serine proteases.
Analysis of the activation status of Akt, NFkappaB, and Stat3 in human diffuse gliomas.
Schick N, Oakeley EJ, Hynes NE, Badache A
The Journal of biological chemistry 2004 Sep 10;279(37):38787-96
The Journal of biological chemistry 2004 Sep 10;279(37):38787-96
Proteolytic conversion of STAT3alpha to STAT3gamma in human neutrophils: role of granule-derived serine proteases.
Kato T, Sakamoto E, Kutsuna H, Kimura-Eto A, Hato F, Kitagawa S
The Journal of biological chemistry 2004 Jul 23;279(30):31076-80
The Journal of biological chemistry 2004 Jul 23;279(30):31076-80
Analysis of the activation status of Akt, NFkappaB, and Stat3 in human diffuse gliomas.
Wang H, Wang H, Zhang W, Huang HJ, Liao WS, Fuller GN
Laboratory investigation; a journal of technical methods and pathology 2004 Aug;84(8):941-51
Laboratory investigation; a journal of technical methods and pathology 2004 Aug;84(8):941-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of STAT3 (phospho Y705) polyclonal antibody (Cat # PAB0616) in mouse liver tissue lysate (35 ug/lane). Phospho-STAT3 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT3 (phospho Y705) polyclonal antibody (Cat # PAB0616) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
