AM00148PU-N

antibody from Acris Antibodies GmbH

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00148PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00148PU-N, RRID:AB_1007500

Product name

anti STAT3 pTyr705 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopeptide conjuagted to KLH. Epitope: Phosphotyrosine 705 ...A P pY L K...

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

9E12

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HEPG2 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab STAT3-9E12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).