Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- IC1799P - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat STAT3 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. The unconjugated antibody (Catalog # MAB1799) detects human, mouse, and rat STAT3 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antigen sequence
P40763- Isotype
- IgG
- Antibody clone number
- 232209
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line. STAT3 knockout HeLa human cervical epithelial cell line was stained with PE-conjugated Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
