PAB28498

antibody from Abnova Corporation

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28498

Provider

Abnova Corporation

Product name

STAT6 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant STAT6

Antigen sequence

VYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMP
PSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLL
CSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLP
PTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQ
SGISMSHMDLR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with STAT6 polyclonal antibody at 1:100-1:250 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with STAT6 polyclonal antibody at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with STAT6 polyclonal antibody (Cat # PAB28498) at 1-4 ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder with STAT6 polyclonal antibody (Cat # PAB28498) shows strong cytoplasmic and nuclear positivity in urothelial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)