AF2167

antibody from R&D Systems

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2167

Provider

R&D Systems

Product name

Human/Mouse/Rat STAT6 aa 627-838 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat STAT6 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Absence of Tumor Necrosis Factor Supports Alternative Activation of Macrophages in the Liver after Infection with Leishmania major.

Hu S, Marshall C, Darby J, Wei W, Lyons AB, Körner H
Frontiers in immunology 2018;9:1

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse STAT6 aa 627-838 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line untreated (-) or treated (+) with 25 μg Recombinant Mouse IL-4 (Catalog # 404-ML) for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat STAT6 Polyclonal Antibody (Catalog # AF2167, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT6 aa 627-838 at approximately 100 - 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of STAT6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat STAT6 aa 627-838 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2167, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.