AM03112PU-N

antibody from Acris Antibodies GmbH

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AM03112PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM03112PU-N, RRID:AB_1624643

Product name

anti ZAP70

Antibody type

Monoclonal

Antigen

Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

ZAP-03

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line. Lane 1, 2, 3, 4: immunostaining with dilution range of anti-ZAP70 (ZAP-03; 4 μg/ml (1), 2 μg/ml (2), 1 μg/ml (3), 0.5 μg/ml (4). Lane 5: immunostaining with anti-ZAP70 comparative antibody. Lane 6: immunostaining with Isotype mouse IgG1 control (PPV-06; AM03095PU-N).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP70 (ZAP-03; 0.5 μg/ml). Lane 3, 4: immunostaining with Isotype mouse IgG1 control (PPV-06; AM03095PU-N)