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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ARG51657 - Provider product page
- Provider
- Arigo
- Product name
- anti-Zap-70 phospho (Tyr319) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from Jurkat cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: methanol-fixed HeLa cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
