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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-12624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Zap-70 Monoclonal Antibody (2F3.2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12624 targets ZAP-70 in IHC (P) and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 2F3.2
- Concentration
- 0.2 mg/mL
Submitted references Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70.
Gladkikh AA, Potashnikova DM, Tatarskiy V Jr, Yastrebova M, Khamidullina A, Barteneva N, Vorobjev I
Cancer medicine 2017 Dec;6(12):2984-2997
Cancer medicine 2017 Dec;6(12):2984-2997
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of ZAP-70 using ZAP-70 Monoclonal Antibody (Product # MA5-12624) on Jurkat Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2) and Ramos (Lane 3). The blot was probed with ZAP70 Mouse Monoclonal Antibody (Product # MA5-12624, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 69 kDa band corresponding to ZAP70 was observed in Jurkat and MOLT-4 cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-Fixed, paraffin-embedded human tonsil stained with anti-Zap-70 using peroxidase-conjugate and AEC chromogen. Note staining of T-Cells.
