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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB11109 - Provider product page
- Provider
- Abnova Corporation
- Product name
- EGFR monoclonal antibody, clone AT2H8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant EGFR.
- Isotype
- IgG
- Antibody clone number
- AT2H8
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Cell extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with EGFR monoclonal antibody, clone AT2H8 (Cat # MAB11109) (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. EGFR monoclonal antibody, clone AT2H8 (Cat # MAB11109) (1:250) was stained with alexa 488 (green).
- Validation comment
- Immunofluorescence
