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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- V2107 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EGFR Antibody
- Antibody type
- Monoclonal
- Antigen
- Purified EGFR from A431 cells was used as the immunogen for this antibody. (1)
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- H9B4
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references EGFR and HER2 levels are frequently elevated in colon cancer cells
Activated EGFR stimulates MUC1 expression in human uterine and pancreatic cancer cell lines.
The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation.
Effects of anti-EGFR antibody cetuximab on androgen-independent prostate cancer cells.
Microarray analyses in bladder cancer cells: inhibition of hTERT expression down-regulates EGFR.
Glycosylation-induced conformational modification positively regulates receptor-receptor association: a study with an aberrant epidermal growth factor receptor (EGFRvIII/DeltaEGFR) expressed in cancer cells.
Targeted expression of a dominant negative epidermal growth factor receptor in the mammary gland of transgenic mice inhibits pubertal mammary duct development.
Epidermal growth factor receptor synthesis is stimulated by phorbol ester and epidermal growth factor. Evidence for a common mechanism.
Li S, Buchbinder E, Wu L, Bjorge J, Fujita D, Zhu S
Discoveries Reports 2014 February;1(1)
Discoveries Reports 2014 February;1(1)
Activated EGFR stimulates MUC1 expression in human uterine and pancreatic cancer cell lines.
Neeraja Dharmaraj, Engel BJ, Carson DD
Journal of cellular biochemistry 2013 Oct;114(10):2314-22
Journal of cellular biochemistry 2013 Oct;114(10):2314-22
The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation.
Danysh BP, Constantinou PE, Lukianova-Hleb EY, Lapotko DO, Carson DD
Theranostics 2012;2(8):777-87
Theranostics 2012;2(8):777-87
Effects of anti-EGFR antibody cetuximab on androgen-independent prostate cancer cells.
Dhupkar P, Dowling M, Cengel K, Chen B
Anticancer research 2010 Jun;30(6):1905-10
Anticancer research 2010 Jun;30(6):1905-10
Microarray analyses in bladder cancer cells: inhibition of hTERT expression down-regulates EGFR.
Kraemer K, Schmidt U, Fuessel S, Herr A, Wirth MP, Meye A
International journal of cancer 2006 Sep 15;119(6):1276-84
International journal of cancer 2006 Sep 15;119(6):1276-84
Glycosylation-induced conformational modification positively regulates receptor-receptor association: a study with an aberrant epidermal growth factor receptor (EGFRvIII/DeltaEGFR) expressed in cancer cells.
Fernandes H, Cohen S, Bishayee S
The Journal of biological chemistry 2001 Feb 16;276(7):5375-83
The Journal of biological chemistry 2001 Feb 16;276(7):5375-83
Targeted expression of a dominant negative epidermal growth factor receptor in the mammary gland of transgenic mice inhibits pubertal mammary duct development.
Xie W, Paterson AJ, Chin E, Nabell LM, Kudlow JE
Molecular endocrinology (Baltimore, Md.) 1997 Nov;11(12):1766-81
Molecular endocrinology (Baltimore, Md.) 1997 Nov;11(12):1766-81
Epidermal growth factor receptor synthesis is stimulated by phorbol ester and epidermal growth factor. Evidence for a common mechanism.
Bjorge JD, Kudlow JE
The Journal of biological chemistry 1987 May 15;262(14):6615-22
The Journal of biological chemistry 1987 May 15;262(14):6615-22
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A431 lysate using EGFR antibody at 1ug/ml.
