ABIN362026

antibody from antibodies-online

Targeting: EGFR ERBB, ERBB1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362026

Provider

antibodies-online

Product name

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1070) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer1070 (R-Y-S (p) -S-D) derived from Human EGFR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pSer1070

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Ca2+/calmodulin-dependent kinase II phosphorylates the epidermal growth factor receptor on multiple sites in the cytoplasmic tail and serine 744 within the kinase domain to regulate signal generation.

Feinmesser RL, Wicks SJ, Taverner CJ, Chantry A
The Journal of biological chemistry 1999 Jun 4;274(23):16168-73

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Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor.

Gamou S, Shimizu N
FEBS letters 1995 Jan 3;357(2):161-4

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Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism.

Gamou S, Shimizu N
Journal of cellular physiology 1994 Jan;158(1):151-9

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Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo.

Heisermann GJ, Gill GN
The Journal of biological chemistry 1988 Sep 15;263(26):13152-8