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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-13048 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGFR Monoclonal Antibody (EGFR.1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13048 targets Epidermal Growth Factor Receptor in FACS, IF, IHC (F), and IP applications and shows reactivity with Equine and Human samples.
- Antibody clone number
- EGFR.1
- Concentration
- 0.2 mg/mL
Submitted references Deep and high-resolution three-dimensional tracking of single particles using nonlinear and multiplexed illumination.
Clinicopathological and extensive immunohistochemical study of a type II pleuropulmonary blastoma.
High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.
Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling.
Optical nanomanipulations of malignant cells: controlled cell damage and fusion.
Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer.
A chimeric pre-ubiquitinated EGF receptor is constitutively endocytosed in a clathrin-dependent, but kinase-independent manner.
The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2.
Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5.
Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR.
The juxtamembrane region of the EGF receptor functions as an activation domain.
Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2.
Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells.
Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane.
Molecular classification system identifies invasive breast carcinoma patients who are most likely and those who are least likely to achieve a complete pathologic response after neoadjuvant chemotherapy.
Effects of cisplatin, alpha-interferon, and 13-cis retinoic acid on the expression of Fas (CD95), intercellular adhesion molecule-1 (ICAM-1), and epidermal growth factor receptor (EGFR) in oral cancer cell lines.
Both clathrin-positive and -negative coats are involved in endosomal sorting of the EGF receptor.
Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits.
Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2.
Retention of intrinsic stem cell hierarchies in carcinoma-derived cell lines.
Abnormal beta-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features.
Novel prognostic value of nuclear epidermal growth factor receptor in breast cancer.
The inhibitory effect of ErbB2 on epidermal growth factor-induced formation of clathrin-coated pits correlates with retention of epidermal growth factor receptor-ErbB2 oligomeric complexes at the plasma membrane.
Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation.
Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer.
Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis.
Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis.
Ligand-independent dimer formation of epidermal growth factor receptor (EGFR) is a step separable from ligand-induced EGFR signaling.
The src homology 2 and phosphotyrosine binding domains of the ShcC adaptor protein function as inhibitors of mitogenic signaling by the epidermal growth factor receptor.
Perillo EP, Liu YL, Huynh K, Liu C, Chou CK, Hung MC, Yeh HC, Dunn AK
Nature communications 2015 Jul 29;6:7874
Nature communications 2015 Jul 29;6:7874
Clinicopathological and extensive immunohistochemical study of a type II pleuropulmonary blastoma.
Yu L, Cheng H, Yang SJ
Fetal and pediatric pathology 2014 Feb;33(1):1-8
Fetal and pediatric pathology 2014 Feb;33(1):1-8
High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.
Minai L, Yeheskely-Hayon D, Yelin D
Scientific reports 2013;3:2146
Scientific reports 2013;3:2146
Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling.
Oksvold MP, Pedersen NM, Forfang L, Smeland EB
FEBS letters 2012 Oct 19;586(20):3575-81
FEBS letters 2012 Oct 19;586(20):3575-81
Optical nanomanipulations of malignant cells: controlled cell damage and fusion.
Minai L, Yeheskely-Hayon D, Golan L, Bisker G, Dann EJ, Yelin D
Small (Weinheim an der Bergstrasse, Germany) 2012 Jun 11;8(11):1732-9
Small (Weinheim an der Bergstrasse, Germany) 2012 Jun 11;8(11):1732-9
Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer.
Freeman DJ, McDorman K, Ogbagabriel S, Kozlosky C, Yang BB, Doshi S, Perez-Ruxio JJ, Fanslow W, Starnes C, Radinsky R
Molecular cancer 2012 Jul 25;11:47
Molecular cancer 2012 Jul 25;11:47
A chimeric pre-ubiquitinated EGF receptor is constitutively endocytosed in a clathrin-dependent, but kinase-independent manner.
Bertelsen V, Sak MM, Breen K, Rødland MS, Johannessen LE, Traub LM, Stang E, Madshus IH
Traffic (Copenhagen, Denmark) 2011 Apr;12(4):507-20
Traffic (Copenhagen, Denmark) 2011 Apr;12(4):507-20
The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2.
Zeineldin R, Ning Y, Hudson LG
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Jun;58(6):529-41
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Jun;58(6):529-41
Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5.
Wegner CS, Malerød L, Pedersen NM, Progida C, Bakke O, Stenmark H, Brech A
Histochemistry and cell biology 2010 Jan;133(1):41-55
Histochemistry and cell biology 2010 Jan;133(1):41-55
Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR.
Al-Nedawi K, Meehan B, Kerbel RS, Allison AC, Rak J
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3794-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3794-9
The juxtamembrane region of the EGF receptor functions as an activation domain.
Red Brewer M, Choi SH, Alvarado D, Moravcevic K, Pozzi A, Lemmon MA, Carpenter G
Molecular cell 2009 Jun 26;34(6):641-51
Molecular cell 2009 Jun 26;34(6):641-51
Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2.
Pedersen NM, Breen K, Rødland MS, Haslekås C, Stang E, Madshus IH
Molecular cancer research : MCR 2009 Feb;7(2):275-84
Molecular cancer research : MCR 2009 Feb;7(2):275-84
Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells.
Barr DJ, Ostermeyer-Fay AG, Matundan RA, Brown DA
Journal of cell science 2008 Oct 1;121(Pt 19):3155-66
Journal of cell science 2008 Oct 1;121(Pt 19):3155-66
Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane.
Offterdinger M, Bastiaens PI
Traffic (Copenhagen, Denmark) 2008 Jan;9(1):147-55
Traffic (Copenhagen, Denmark) 2008 Jan;9(1):147-55
Molecular classification system identifies invasive breast carcinoma patients who are most likely and those who are least likely to achieve a complete pathologic response after neoadjuvant chemotherapy.
Goldstein NS, Decker D, Severson D, Schell S, Vicini F, Margolis J, Dekhne NS
Cancer 2007 Oct 15;110(8):1687-96
Cancer 2007 Oct 15;110(8):1687-96
Effects of cisplatin, alpha-interferon, and 13-cis retinoic acid on the expression of Fas (CD95), intercellular adhesion molecule-1 (ICAM-1), and epidermal growth factor receptor (EGFR) in oral cancer cell lines.
Sundelin K, Roberg K, Grénman R, Håkansson L
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2007 Mar;36(3):177-83
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2007 Mar;36(3):177-83
Both clathrin-positive and -negative coats are involved in endosomal sorting of the EGF receptor.
Myromslien FD, Grøvdal LM, Raiborg C, Stenmark H, Madshus IH, Stang E
Experimental cell research 2006 Oct 1;312(16):3036-48
Experimental cell research 2006 Oct 1;312(16):3036-48
Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits.
Johannessen LE, Pedersen NM, Pedersen KW, Madshus IH, Stang E
Molecular and cellular biology 2006 Jan;26(2):389-401
Molecular and cellular biology 2006 Jan;26(2):389-401
Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2.
Longva KE, Pedersen NM, Haslekås C, Stang E, Madshus IH
International journal of cancer 2005 Sep 1;116(3):359-67
International journal of cancer 2005 Sep 1;116(3):359-67
Retention of intrinsic stem cell hierarchies in carcinoma-derived cell lines.
Locke M, Heywood M, Fawell S, Mackenzie IC
Cancer research 2005 Oct 1;65(19):8944-50
Cancer research 2005 Oct 1;65(19):8944-50
Abnormal beta-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features.
Odajima T, Sasaki Y, Tanaka N, Kato-Mori Y, Asanuma H, Ikeda T, Satoh M, Hiratsuka H, Tokino T, Sawada N
Human pathology 2005 Mar;36(3):234-41
Human pathology 2005 Mar;36(3):234-41
Novel prognostic value of nuclear epidermal growth factor receptor in breast cancer.
Lo HW, Xia W, Wei Y, Ali-Seyed M, Huang SF, Hung MC
Cancer research 2005 Jan 1;65(1):338-48
Cancer research 2005 Jan 1;65(1):338-48
The inhibitory effect of ErbB2 on epidermal growth factor-induced formation of clathrin-coated pits correlates with retention of epidermal growth factor receptor-ErbB2 oligomeric complexes at the plasma membrane.
Haslekås C, Breen K, Pedersen KW, Johannessen LE, Stang E, Madshus IH
Molecular biology of the cell 2005 Dec;16(12):5832-42
Molecular biology of the cell 2005 Dec;16(12):5832-42
Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation.
Grøvdal LM, Stang E, Sorkin A, Madshus IH
Experimental cell research 2004 Nov 1;300(2):388-95
Experimental cell research 2004 Nov 1;300(2):388-95
Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer.
Witton CJ, Reeves JR, Going JJ, Cooke TG, Bartlett JM
The Journal of pathology 2003 Jul;200(3):290-7
The Journal of pathology 2003 Jul;200(3):290-7
Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis.
Ge H, Gong X, Tang CK
International journal of cancer 2002 Mar 20;98(3):357-61
International journal of cancer 2002 Mar 20;98(3):357-61
Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis.
Ge H, Gong X, Tang CK
International journal of cancer 2002 Mar 20;98(3):357-61
International journal of cancer 2002 Mar 20;98(3):357-61
Ligand-independent dimer formation of epidermal growth factor receptor (EGFR) is a step separable from ligand-induced EGFR signaling.
Yu X, Sharma KD, Takahashi T, Iwamoto R, Mekada E
Molecular biology of the cell 2002 Jul;13(7):2547-57
Molecular biology of the cell 2002 Jul;13(7):2547-57
The src homology 2 and phosphotyrosine binding domains of the ShcC adaptor protein function as inhibitors of mitogenic signaling by the epidermal growth factor receptor.
O'Bryan JP, Lambert QT, Der CJ
The Journal of biological chemistry 1998 Aug 7;273(32):20431-7
The Journal of biological chemistry 1998 Aug 7;273(32):20431-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Mouse Monoclonal Antibody (Product # MA5-13048) at a concentration of 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13048) on Native Human A431 Cells.
