MA5-13266

antibody from Invitrogen Antibodies

Targeting: EGFR ERBB, ERBB1

Antibody data

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Validation data

Product number: MA5-13266 - Provider product page
Provider: Invitrogen Antibodies
Product name: EGFR Monoclonal Antibody (111.6), Biotin
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-13266 targets Epidermal Growth Factor Receptor in IHC (P), IP, and WB applications and shows reactivity with Human samples.
Conjugate: Biotin
Antibody clone number: 111.6
Concentration: 0.2 mg/mL

EGFR protein structure - MA5-13266 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13266) on A431 Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13266) on A431 Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2) and Jurkat (Lane 3). The blot was probed with Anti-EGFR Monoclonal Antibody, Biotin (Product # MA5-13266, 1 µg/mL) and detected by chemiluminescence using Poly-HRP Streptavidin (Product# N200, 1:10,000 dilution). A 170 kDa band corresponding to EGFR was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of EGFR was achieved by transfecting A431 cells with EGFR specific validated siRNA (Silencer® select Cat # s563, s564 and s565). Western blot analysis (Fig a) was performed using membrane enriched extracts from the EGFR knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-EGFR Monoclonal Antibody (111.6), Biotin (Product# MA5-13266, 1-3 µg/mL) and Poly-HRP Streptavidin (Product# N200, 1:10,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to EGFR.
Conjugate: Biotin