Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- MA5-15643 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAK Monoclonal Antibody (10H7A6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15643 targets FAK in WB applications and shows reactivity with Human samples.
- Antibody clone number
- 10H7A6
- Concentration
- Conc. Not Determined
Submitted references Distinct PKA regulatory subunits mediate PGE(2) inhibition of TGFβ-1-stimulated collagen I translation and myofibroblast differentiation.
Wettlaufer SH, Penke LR, Okunishi K, Peters-Golden M
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAK using FAK monoclonal antibody (Product # MA5-15643) in FAK (AA: 354-533) human IgG Fc transfected HEK293 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. PKA subunit RIIbeta agonist inhibits TGFbeta-1-stimulated phosphorylation of FAK and relative expression of SRF. A and B : reduced level of phosphorylation of FAK by PKARIIbeta agonist (Sp-5,6-DCI-cBIMPs) ( B ) but not PKARIbeta agonist (2-Cl-8-MA-cAMP) ( A ). After 24 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for Western blot analysis. Blots are from one representative experiment, and graph shows mean and SE values from densitometric analysis of n = 4 independent experiments for p-FAK. *Significant difference from TGFbeta-1 alone, P < 0.001. C and D : inhibition of SRF mRNA ( C ) and protein ( D ) expression by PKARIIbeta agonist. After 48 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for mRNA analysis by qPCR and protein analysis by Western blot. The graph shows mean and SE values from relative expression of n = 4 independent experiments. Densitometry of SRF protein blots was normalized to GAPDH. Relative expression of SRF was normalized to GAPDH. E : effect of R isoform-specific knockdown on PGE 2 inhibition of FAK protein phosphorylation by TGFbeta-1 After incubation for 4 days with R isoform-specific siRNA, HLFs were treated for 30 min with or without PGE 2 (500 nM) before addit