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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ABIN1504512 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Serum
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western Blot Analysis: Lanes 1 and 2 demonstarate untransformed and Src (527F)-transformed Rat2 cells(lanes 1 and 2, respectively) Lanes 3-6 demonstrate untreated (lanes 3 and 5) and pervanadate-treated (lanes 4 and 6) C2C12 (lanes 3 and 4) and HUVEC (lanes 5 and 6) cell lysate resolved by SDS-PAGE, transferred to PVDF, and probed with anti phospho-FAK (Tyr576) (1:500) Proteins were visualized using a donkey anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates FAK (~125kDa).
