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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
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- Product number
- ABIN307050 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 34Q36
- Vial size
- 100 μg
- Concentration
- 1 mg/ml
- Storage
- -20°C
Submitted references Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase.
Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. Role of Src family kinases and Rho family GTPases.
Phosphorylation of focal adhesion kinase at tyrosine 861 is crucial for Ras transformation of fibroblasts.
Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive.
Focal adhesion kinase: at the crossroads of signal transduction.
pp125FAK in the focal adhesion.
Signal transduction from the extracellular matrix--a role for the focal adhesion protein-tyrosine kinase FAK.
Cao Y, Baig MR, Hamm LL, Wu K, Simon EE
Biochemical and biophysical research communications 2005 Mar 11;328(2):560-6
Biochemical and biophysical research communications 2005 Mar 11;328(2):560-6
Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. Role of Src family kinases and Rho family GTPases.
Lunn JA, Rozengurt E
The Journal of biological chemistry 2004 Oct 22;279(43):45266-78
The Journal of biological chemistry 2004 Oct 22;279(43):45266-78
Phosphorylation of focal adhesion kinase at tyrosine 861 is crucial for Ras transformation of fibroblasts.
Lim Y, Han I, Jeon J, Park H, Bahk YY, Oh ES
The Journal of biological chemistry 2004 Jul 9;279(28):29060-5
The Journal of biological chemistry 2004 Jul 9;279(28):29060-5
Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive.
Romanova LY, Hashimoto S, Chay KO, Blagosklonny MV, Sabe H, Mushinski JF
Journal of cell science 2004 Aug 1;117(Pt 17):3759-68
Journal of cell science 2004 Aug 1;117(Pt 17):3759-68
Focal adhesion kinase: at the crossroads of signal transduction.
Ilić D, Damsky CH, Yamamoto T
Journal of cell science 1997 Feb;110 ( Pt 4):401-7
Journal of cell science 1997 Feb;110 ( Pt 4):401-7
pp125FAK in the focal adhesion.
Otey CA
International review of cytology 1996;167:161-83
International review of cytology 1996;167:161-83
Signal transduction from the extracellular matrix--a role for the focal adhesion protein-tyrosine kinase FAK.
Schlaepfer DD, Hunter T
Cell structure and function 1996 Oct;21(5):445-50
Cell structure and function 1996 Oct;21(5):445-50
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Proteins from cell extracts of human HeLa cells (lane 1), mouse L929 cells (lane 2), and rat PC12 cells (lane 3) were resolved by SDS- PAGE and transferred to PVDF. The membranes were incubated with this FAK monoclonal antibody (clone 34Q36) at a concentration of 1 ?g/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(ab?)2 anti-mouse IgG alkaline phosphatase conjugated antibody and Kodak BioMax film.
