LS-B4416

antibody from LSBio

Targeting: SHC1 p66, SHC, ShcA

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B4416

Provider

LSBio

Product name

IHC-plus™ p66 / SHC Antibody (clone 3F4) LS-B4416

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3F4

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

Proximity ligation assay

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.