sc-288

antibody from Santa Cruz Biotechnology

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-288

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-288, RRID:AB_632395

Product name

Anti-SHC1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches.

Licciulli S, Luise C, Zanardi A, Giorgetti L, Viale G, Lanfrancone L, Carbone R, Alcalay M
BMC cell biology 2010 Jan 20;11:5

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Novel target for induction of apoptosis by cyclo-oxygenase-2 inhibitor SC-236 through a protein kinase C-β₁-dependent pathway

Xiao-Hua Jiang, Shiu-Kum Lam, Marie CM Lin, Shi-Hu Jiang, Hsiang-Fu Kung, Eric D Slosberg, Jae Won Soh, I Bernard Weinstein, Benjamin Chun-Yu Wong
Oncogene 2002 Sep;21(39):6113-6122

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 63

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.