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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM00144PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00144PU-N, RRID:AB_1007315
- Product name
- anti SHC1 / SHC pSer36 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic phosphopeptide conjugated to KLHEpitope: E-L-P-pS-P-S-A
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6E10
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis: HeLa cells were cultured under serum-free conditions for 24h and subsequently stimulated with 10 ng/ml EGF. Cells were lysed with RIPA buffer and shc immunoprecipitated with polyclonal anti-shc (Transduction Labs). Immunoprecipitates were separated by SDS-PAGE. Immunoblots were developed using mab shc/p66-6E10 at 1 µg/ml.
