644304

antibody from BioLegend

Targeting: SYK

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

644304

Provider

BioLegend

Proper citation

BioLegend Cat#644304, RRID:AB_2565304

Product name

PE anti-Syk

Antibody type

Monoclonal

Antigen

Human Syk peptide aa 314-339

Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

4D10.2

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were surface stained with CD19 APC then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with anti-Syk (clone 4D10.2) PE (top) or mouse IgG2a, ?? PE isotype control (bottom).

Conjugate

Yellow dye