ABIN362477

antibody from antibodies-online

Targeting: SYK

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362477

Provider

antibodies-online

Product name

anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pTyr323 (N-P-Y (p) -E- P) derived from Human syk. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human

Host

Rabbit

Epitope

pTyr323

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Phosphorylation of Syk activation loop tyrosines is essential for Syk function. An in vivo study using a specific anti-Syk activation loop phosphotyrosine antibody.

Zhang J, Billingsley ML, Kincaid RL, Siraganian RP
The Journal of biological chemistry 2000 Nov 10;275(45):35442-7

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Tyrosine kinase SYK: essential functions for immunoreceptor signalling.

Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL
Immunology today 2000 Mar;21(3):148-54

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Coordinated regulation of the tyrosine phosphorylation of Cbl by Fyn and Syk tyrosine kinases.

Deckert M, Elly C, Altman A, Liu YC
The Journal of biological chemistry 1998 Apr 10;273(15):8867-74

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Phospholipase C-gamma1 interacts with conserved phosphotyrosyl residues in the linker region of Syk and is a substrate for Syk.

Law CL, Chandran KA, Sidorenko SP, Clark EA
Molecular and cellular biology 1996 Apr;16(4):1305-15