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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AP3093a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3093a, RRID:AB_636820
- Product name
- Phospho-ErbB2(Y1112) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-ERBB2-Y1112 Phospho-specific Pab (Cat. #AP3093a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
- Primary Ab dilution
- 1:500
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of extracts from A431 cells, untreated or treated with EGF at 100ng/ml, using phospho-ErBB2 (Y1112) (left) or ErBB2 antibody (right)."
- Primary Ab dilution
- 1:2000
