AM00050PU-N

antibody from Acris Antibodies GmbH

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

AM00050PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00050PU-N, RRID:AB_977170

Product name

anti CD340 / ERBB2 / HER2 pTyr1112 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopetide conjugated to KLH.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

19G5

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: ControlLane 2: 5 min EGFLane 3: 15 min EGFLane 4: 30 min EGFLane 5: 1h EGFLane 6; 2h EGFLane 7: 4h EGFLane 8: 8h EGF