Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00053PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00053PU-N, RRID:AB_977173
- Product name
- anti CD340 / ERBB2 / HER2 pTyr1248
- Antibody type
- Monoclonal
- Antigen
- Phosphopeptide conjugated to hemocyanin
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6G7
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 µg/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF