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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00023071-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00023071-M01, RRID:AB_509183
- Product name
- TXNDC4 monoclonal antibody (M01), clone 3C7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TXNDC4.
- Antigen sequence
EITSLDTENIDEILNNADVALVNFYADWCRFSQML
HPIFEEASDVIKEEFPNENQVVFARVDCDQHSDIA
QRYRISKYPTLKLFRNGMMMKREYRGQRSVKALAD
YIRQQKSDPIQEIRDLAEITTLDRSKRNIIGYFEQ
KDSDNYRVFERVANILHDDCAFLSAFGDVSKPERY
SGDNIIYKPPGHSAPDMVYLGAMTNFDVTYNWIQD
KCVPLVREITFENGEELTEEGLPFLILFHMKEDTE
SLEIFQNEVARQLISEKGTINFLHADCDKFRHPLL
HIQKTPADCPVIAIDSFRHMYVFGDFKDVLIPGKL
KQFVFDLHSGKLHREFHHGPDPTDTAPGEQAQDVA
SSPPESSFQKLAPSEYRYTLLRDRDEL- Isotype
- IgG
- Antibody clone number
- 3C7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TXNDC4 monoclonal antibody (M01), clone 3C7 Western Blot analysis of TXNDC4 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TXNDC4 monoclonal antibody (M01), clone 3C7. Western Blot analysis of TXNDC4 expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TXNDC4 expression in transfected 293T cell line by TXNDC4 monoclonal antibody (M01), clone 3C7.Lane 1: TXNDC4 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TXNDC4 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of TXNDC4 transfected lysate using anti-TXNDC4 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TXNDC4 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TXNDC4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
