Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-201-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL-1 beta , recombinant porcine IL-1 beta , recombinant rat IL-1 beta , recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant cotton rat IL-1 beta , recombinant feline IL-1 beta , and recombinant guinea pig IL-1 beta is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells.
Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration.
Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome.
Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages.
RORα controls inflammatory state of human macrophages.
The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes.
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis.
The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.
An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.
ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells.
Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation.
17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome.
Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS.
Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1β for Caspase-1 Cleavage.
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1α.
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Complement C5a potentiates uric acid crystal-induced IL-1β production.
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes.
ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Cheng R, Billet S, Liu C, Haldar S, Choudhury D, Tripathi M, Hav M, Merchant A, Hu T, Huang H, Zhou H, Bhowmick NA
Oncogene 2020 Feb;39(7):1543-1556
Oncogene 2020 Feb;39(7):1543-1556
Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration.
De Witte C, Demeyere K, De Bruyckere S, Taminiau B, Daube G, Ducatelle R, Meyer E, Haesebrouck F
Veterinary research 2019 May 24;50(1):39
Veterinary research 2019 May 24;50(1):39
Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome.
Botto S, Abraham J, Mizuno N, Pryke K, Gall B, Landais I, Streblow DN, Fruh KJ, DeFilippis VR
mBio 2019 Feb 12;10(1)
mBio 2019 Feb 12;10(1)
Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages.
Goddard PJ, Sanchez-Garrido J, Slater SL, Kalyan M, Ruano-Gallego D, Marchès O, Fernández LÁ, Frankel G, Shenoy AR
Cell reports 2019 Apr 23;27(4):1008-1017.e6
Cell reports 2019 Apr 23;27(4):1008-1017.e6
RORα controls inflammatory state of human macrophages.
Nejati Moharrami N, Bjørkøy Tande E, Ryan L, Espevik T, Boyartchuk V
PloS one 2018;13(11):e0207374
PloS one 2018;13(11):e0207374
The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes.
Kasper L, König A, Koenig PA, Gresnigt MS, Westman J, Drummond RA, Lionakis MS, Groß O, Ruland J, Naglik JR, Hube B
Nature communications 2018 Oct 15;9(1):4260
Nature communications 2018 Oct 15;9(1):4260
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis.
Qiang L, Sample A, Shea CR, Soltani K, Macleod KF, He YY
Autophagy 2017;13(12):2086-2103
Autophagy 2017;13(12):2086-2103
The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.
Hendrawan K, Whittington CM, Brandley MC, Belov K, Thompson MB
Journal of experimental zoology. Part B, Molecular and developmental evolution 2017 Jun;328(4):334-346
Journal of experimental zoology. Part B, Molecular and developmental evolution 2017 Jun;328(4):334-346
An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.
Young HL, Rowling EJ, Bugatti M, Giurisato E, Luheshi N, Arozarena I, Acosta JC, Kamarashev J, Frederick DT, Cooper ZA, Reuben A, Gil J, Flaherty KT, Wargo JA, Vermi W, Smith MP, Wellbrock C, Hurlstone A
The Journal of experimental medicine 2017 Jun 5;214(6):1691-1710
The Journal of experimental medicine 2017 Jun 5;214(6):1691-1710
ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells.
Jeong JH, Kim J, Kim J, Heo HR, Jeong JS, Ryu YJ, Hong Y, Han SS, Hong SH, Lee SJ, Kim WJ
Tuberculosis and respiratory diseases 2017 Jul;80(3):247-254
Tuberculosis and respiratory diseases 2017 Jul;80(3):247-254
Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation.
Primiano MJ, Lefker BA, Bowman MR, Bree AG, Hubeau C, Bonin PD, Mangan M, Dower K, Monks BG, Cushing L, Wang S, Guzova J, Jiao A, Lin LL, Latz E, Hepworth D, Hall JP
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2421-33
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2421-33
17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome.
Cipollina C, Di Vincenzo S, Siena L, Di Sano C, Gjomarkaj M, Pace E
Scientific reports 2016 Nov 24;6:37625
Scientific reports 2016 Nov 24;6:37625
Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS.
Park YH, Wood G, Kastner DL, Chae JJ
Nature immunology 2016 Aug;17(8):914-21
Nature immunology 2016 Aug;17(8):914-21
Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1β for Caspase-1 Cleavage.
Edye ME, Brough D, Allan SM
The Journal of biological chemistry 2015 Oct 16;290(42):25374-81
The Journal of biological chemistry 2015 Oct 16;290(42):25374-81
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
Coll RC, Robertson AA, Chae JJ, Higgins SC, Muñoz-Planillo R, Inserra MC, Vetter I, Dungan LS, Monks BG, Stutz A, Croker DE, Butler MS, Haneklaus M, Sutton CE, Núñez G, Latz E, Kastner DL, Mills KH, Masters SL, Schroder K, Cooper MA, O'Neill LA
Nature medicine 2015 Mar;21(3):248-55
Nature medicine 2015 Mar;21(3):248-55
Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
Sokolowska M, Chen LY, Liu Y, Martinez-Anton A, Qi HY, Logun C, Alsaaty S, Park YH, Kastner DL, Chae JJ, Shelhamer JH
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5472-5487
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5472-5487
Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
Ahn H, Kim J, Lee MJ, Kim YJ, Cho YW, Lee GS
Cytokine 2015 Feb;71(2):223-31
Cytokine 2015 Feb;71(2):223-31
Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
Kortmann J, Brubaker SW, Monack DM
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):815-9
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):815-9
IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1α.
Foucher ED, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
European journal of immunology 2015 Apr;45(4):1092-102
European journal of immunology 2015 Apr;45(4):1092-102
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
Journal of immunology research 2014;2014:593562
Journal of immunology research 2014;2014:593562
Complement C5a potentiates uric acid crystal-induced IL-1β production.
An LL, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
European journal of immunology 2014 Dec;44(12):3669-79
European journal of immunology 2014 Dec;44(12):3669-79
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
Journal of lipid research 2013 Nov;54(11):2998-3008
Journal of lipid research 2013 Nov;54(11):2998-3008
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
Lee GS, Subramanian N, Kim AI, Aksentijevich I, Goldbach-Mansky R, Sacks DB, Germain RN, Kastner DL, Chae JJ
Nature 2012 Dec 6;492(7427):123-7
Nature 2012 Dec 6;492(7427):123-7
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes.
Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4175-82
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4175-82
ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
Cancer research 2008 Nov 15;68(22):9541-50
Cancer research 2008 Nov 15;68(22):9541-50
Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
Cytokine 2008 Dec;44(3):352-60
Cytokine 2008 Dec;44(3):352-60
Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
Biology of reproduction 2007 Jun;76(6):1009-15
Biology of reproduction 2007 Jun;76(6):1009-15
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human IL-1ß/IL-1F2 by Western Blot. Detection of Human IL-1ß/IL-1F2 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-1ß/IL-1F2 in Human Tonsil. IL-1ß/IL-1F2 in Human Tonsil.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1ß/IL-1F2 and Neutral-ization by Human IL-1ß/IL-1F2 Antibody. Cell Proliferation Induced by IL-1ß/IL-1F2 and Neutral-ization by Human IL-1ß/IL-1F2 Antibody.
