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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- TA506443 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506443, RRID:AB_2623768
- Product name
- IL1B mouse monoclonal antibody, clone OTI3E1 (formerly 3E1)
- Antibody type
- Monoclonal
- Description
- IL1B mouse monoclonal antibody, clone OTI3E1 (formerly 3E1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IL1B
- Isotype
- IgG
- Antibody clone number
- OTI3E1
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Functional proteomics revealed IL-1β amplifies TNF downstream protein signals in human synoviocytes in a TNF-independent manner.
Shen S, Guo J, Luo Y, Zhang W, Cui Y, Wang Q, Zhang Z, Wang T
Biochemical and biophysical research communications 2014 Jul 18;450(1):538-44
Biochemical and biophysical research communications 2014 Jul 18;450(1):538-44
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1B (RC202079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1B.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-IL1B mouse monoclonal antibody (TA506443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1B(RC202079).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506443)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry of IL1B protein level by using anti-IL1B antibody in synovial tissues from fracture and OA patients, respectively. Dilution: 1:100
- Validation comment
- IHC
