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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC8406A-025 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-1 beta/IL-1F2 by direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-1 beta/IL-1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes either (A) untreated or (B) treated with 250 ng/mL LPS overnight were stained with Rabbit Anti-Human IL-1 beta/IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC8406A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC105A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
